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KMID : 0603820150210040241
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Journal of Experimental & Biomedical Science
2015 Volume.21 No. 4 p.241 ~ p.247
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Simultaneous Determination of Plasma Lactate, Pyruvate, and Ketone Bodies following tert-Butyldimethylsilyl Derivatization using GC-MS-SIM
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Yoon Hye-Ran
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Abstract
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Lactate and ketone bodies are considered biological markers for ketosis and several inherited metabolic disorders. In the current study, the specific ratios of lactate and ketone bodies as analytical tools for differential diagnosis of various lactic acidosis were devised. The study included a protein precipitation step following tert-butyldimethylsilyl derivatisation. Total run time was approximately 30 min including sample preparation and GS/MS analysis. The limits of detection were below 0.1 pg/mL over the targeted 4 analytes. The calibration curve was linear over the concentration range of 0.001~250 μg/mL for pyruvate, beta-hydroxybutyrate, and acetoacetate (R² > 0.99). Inter-day accuracy and precision were 87.7~94.8% with RSD of 2.5~5.7% at 2 levels. Absolute recoveries (%) of target analytes were 87.0~98.4%. The method was validated for the quantification of lactate and ketone bodies for differentiation of lactic acidosis.
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KEYWORD
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Lactate, Pyruvate, Beta-hydroxybutyrate, Acetoacetate, tert-butyldimethylsilylation
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